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**OBITools** is a set of python programs developed to simplify the manipulation of sequence files in our labs. They were mainly designed to help us for analyzing Next Generation Sequencer outputs (454 or Illumina) in the context of [DNA Metabarcoding](http://metabarcoding.org).
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They are highly related with the following softwares:
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They are highly related to the following softwares:
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- [ecoPCR](http://www.grenoble.prabi.fr/trac/ecoPCR) for [DNA Metabarcoding](http://metabarcoding.org) primers in silico evaluation.
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- [ecoPrimers](http://www.grenoble.prabi.fr/trac/ecoPrimers) for [DNA Metabarcoding](http://metabarcoding.org) primers design.
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- [sumatra and sumaclust](http://metabarcoding.org/sumatra) for fast alignment and clustering of NGS reads.
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- [ecoPCR](https://git.metabarcoding.org/obitools/ecopcr/wikis/home) for [DNA Metabarcoding](http://metabarcoding.org) primers in silico evaluation.
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- [ecoPrimers](https://git.metabarcoding.org/obitools/ecoprimers/wikis/home) for [DNA Metabarcoding](http://metabarcoding.org) primers design.
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- [sumatra](https://git.metabarcoding.org/obitools/sumatra/wikis/home) for fast alignment and [sumaclust](https://git.metabarcoding.org/obitools/sumaclust/wikis/home) for the clustering of NGS reads.
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The programs evolved with our needs. They mainly provide facilities to manipulate sequence files in [Fasta](http://en.wikipedia.org/wiki/FASTA_format) and [FastQ](http://en.wikipedia.org/wiki/FASTQ_format) format. Other input formats like [genbank](http://www.ncbi.nlm.nih.gov/Sitemap/samplerecord.html) or [embl](http://en.wikipedia.org/wiki/European_Nucleotide_Archive) are supported too as well as formats related to [ecoPCR](http://www.grenoble.prabi.fr/trac/ecoPCR).
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