GitLab

Hi,

I'm trying to use ngsfilter to de-multiplex my sequences, but I don't have any tags, just different primers for different samples/amplicons. When I use '--no-tags', my primers can be identified but there's no 'sample' attribute at all. If I don't use '--no-tags' then all my sequences are unidentified. So is there a way to demultiplex using just primers not tags?

The command I used:

obi ngsfilter -t obiRL1/ngsfile -u obiRL1/unidentified_sequences --no-tags -e 1 obiRL1/good_sequences obiRL1/identified_sequences

My ngsfilter file:

Dookie 1 -:- TCTGATHTGGATHTAYG ACRTTRCCRGGHGCYT F @ Dookie 2 -:- GCTTTCCCACGAATAAAT ATRAARTTTACWGCT F @ Dookie 3 -:- TTCGGBGARTCVG TTCTCBGANGTCATRTG F @ Dookie 4 -:- GTGGAYGCBAARACVA TTCCCATBAGDATRT F @ Dookie 5 -:- GCCTGYGARATHCAR GCRAABACCATVACG F @

Any comments are welcomed, thank you!