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Hi there,

I am currently trying to build a trnL reference library from the EMBL Release 130 for a plant metabarcoding project. I have been trying to follow the tutorial outlined on the Obitools webpage but I've been running into several issues.

One issue I'm having is with the taxonomy assignment using the ecoTag function. Every time I try to run the ecoTag program, it only goes to about 74% completion and then the time reads out 00:00:00 and the cache size is printed. I've tried increasing the cache size by several orders of magnitude but to no avail. Am I missing something in what could be causing this part of the program to hang?

Code below:

ecotag -d ./GlobalRef/EMBL_Taxo/R130/embl_r130 -R ./GlobalRef/gh_database_r130.fasta --sort=count -m 0.98 -r physeq_merged_count_sort.fasta > physeq_merged_count_sort_ecotagGlobal_r130.fasta