ecoprimers finally return nothing for primers
I am trying to design primers for Influenza A virus (taxid:11320), the input database containing 3 viral species output by obiconvert. The ecoPrimers (v0.5) failed to design primers for Influenza A virus. The codes are as follows:
obiconvert --genbank -t $taxdmp_path --ecopcrdb-output=$ecodb $test_gb
ecoPrimers -d $ecodb -l 500 -L 2000 -r 11320 > $reportThe output is as follows:
#
# ecoPrimer version 0.5
# Rank level optimisation : species
# max error count by oligonucleotide : 3
#
# Restricted to taxon:
# 11320 : Influenza A virus (species)
#
# strict primer quorum : 0.70
# example quorum : 0.90
# counterexample quorum : 0.10
#
# database : /nfs-test/lhs/virus_primer/ecodb/ecodb
# Database is constituted of 56 examples corresponding to 1 species
# and 0 counterexamples corresponding to 0 species
#
# amplifiat length between [500,2000] bp
# DB sequences are considered as linear
# Pairs having specificity less than 0.60 will be ignored
#How can I fix this problem? Any answers are appreciated.