Commit ee54019d authored by Eric Coissac's avatar Eric Coissac

Add annotation of nuclear rDNA cistron

parent 7f75da85
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#!/bin/bash
#
# Annotate the Intergenic Spacer (ITS) of nuclear rDNA cluster
#
#========================================================================================
#
# This script is based on ITSx
#
#
# go_its.sh <FASTAFILE>
#
# - <FASTAFILE> : The fasta file containing the cluster to annotate
#
# Results are printed to the standart output
#
#========================================================================================
# -- CAUTION -- Works as long than the script
# is not called through a symlink
THIS_DIR="$(dirname ${BASH_SOURCE[0]})"
source "${THIS_DIR}/../../../scripts/bash_init.sh"
pushTmpDir ORG.its
loginfo "Normalizing nuclear rDNA cistron..."
RRNADB="${NUCRRNA_DATA_DIR}/plants/nuc_RRNA.hmm"
if [[ ! "$1" =~ ^/ ]]; then
QUERY="${CALL_DIR}/$1"
else
QUERY="$1"
fi
strand=( $(hmmsearch --max ${RRNADB} ${QUERY} | \
$AwkCmd '/Query: / { \
profil=$2; \
match($3,"[0-9][0-9]*");\
lprof=substr($3,RSTART,RLENGTH)} \
/ [0-9][0-9]* ! / { \
print profil,lprof,$7,$8,$10,$11}' | \
$AwkCmd '($3 <=5) && (($2-$4) <=5) { \
full=1;$5=$5-$3+1;$6=$6+($2-$4)} \
{loc="Forward"} \
($1 ~ /_RC$/) { \
loc="Reverse"} \
(full==1) {match($1,"_..*S");\
rrna=substr($1,RSTART+1,RLENGTH-1);\
print loc;\
full=0
}' | sort | uniq) )
if [[ "${#strand[@]}" == 1 ]] ; then
if [[ "${strand[0]}" == "Forward" ]] ; then
cat ${QUERY}
else
fastarevcomp -f ${QUERY}
fi
else
logerror "Cannot determine the Cistron orientation"
exit 1
fi
loginfo "Done."
popTmpDir
exit 0
#!/bin/bash
#
# Annotate the Intergenic Spacer (ITS) of nuclear rDNA cluster
#
#========================================================================================
#
# This script is based on ITSx
#
#
# go_its.sh <FASTAFILE>
#
# - <FASTAFILE> : The fasta file containing the cluster to annotate
#
# Results are printed to the standart output
#
#========================================================================================
# -- CAUTION -- Works as long than the script
# is not called through a symlink
THIS_DIR="$(dirname ${BASH_SOURCE[0]})"
source "${THIS_DIR}/../../../scripts/bash_init.sh"
pushTmpDir ORG.its
loginfo "Annotating ITS and TSU..."
RRNADB="${NUCRRNA_DATA_DIR}/plants/nuc_RRNA.hmm"
if [[ ! "$1" =~ ^/ ]]; then
QUERY="${CALL_DIR}/$1"
else
QUERY="$1"
fi
ITSx -p "${ITS_DATA_DIR}/ITSx_db/HMMs" -i "${QUERY}" -o "output.itsx"
ITS1=( $(sed -E 's/.*ITS1: *([0-9]+)-([0-9]+).*/\1 \2/' "output.itsx.positions.txt" ) )
ITS2=( $(sed -E 's/.*ITS2: *([0-9]+)-([0-9]+).*/\1 \2/' "output.itsx.positions.txt" ) )
TSU=( $(sed -E 's/.*5\.8S: *([0-9]+)-([0-9]+).*/\1 \2/' "output.itsx.positions.txt" ) )
if [[ ${#ITS1[@]}=="2" ]] ; then
echo "FT misc_RNA ${ITS1[0]}..${ITS1[1]}"
echo 'FT /gene="ITS1"'
echo 'FT /note="internal transcribed spacer 1, ITS1"'
fi
if [[ ${#TSU[@]}=="2" ]] ; then
echo "FT rRNA ${TSU[0]}..${TSU[1]}"
echo 'FT /gene="5.8S rRNA"'
echo 'FT /product="5.8S ribosomal nuclear RNA"'
fi
if [[ ${#ITS2[@]}=="2" ]] ; then
echo "FT misc_RNA ${ITS2[0]}..${ITS2[1]}"
echo 'FT /gene="ITS2"'
echo 'FT /note="internal transcribed spacer 2, ITS2"'
fi
hmmsearch --max ${RRNADB} ${QUERY} | \
$AwkCmd '/Query: / { \
profil=$2; \
match($3,"[0-9][0-9]*");\
lprof=substr($3,RSTART,RLENGTH)} \
/ [0-9][0-9]* ! / { \
print profil,lprof,$7,$8,$10,$11}' | \
$AwkCmd '($3 <=5) && (($2-$4) <=5) { \
full=1;$5=$5-$3+1;$6=$6+($2-$4)} \
{loc=$5".."$6} \
($1 ~ /_RC$/) { \
loc="complement("loc")"} \
(full==1) {match($1,"_..*S");\
rrna=substr($1,RSTART+1,RLENGTH-1);\
print "FT rRNA " loc; \
print "FT /gene=\"rrn"rrna"\""
print "FT /product=\""rrna" ribosomal RNA\"";\
full=0
}'
loginfo "Done."
popTmpDir
exit 0
......@@ -23,9 +23,41 @@ taxid="no"
normalization="yes"
irdetection="yes"
organism="no"
types="chloro"
function usage {
echo "Usage:" ;
echo " $1 "'[-t|--ncbi-taxid ###] [-n|--no-normalization] \'
echo ' [-i|--no-ir-detection] [-h|--help] \ '
echo ' [-o|--organism <organism_name>] \ '
echo ' [-c|--chloroplast|-r|--nuclear-rdna|-m|--mitochondrion] <FASTAFILE>'
echo
echo "Options:"
echo ' -t ### | --ncbi-taxid ###'
echo ' ### represents the ncbi taxid associated to the sequence'
echo
echo ' -i | --no-ir-detection'
echo ' Does not look for inverted repeats in the plastid genome'
echo
echo ' -o | --organism <organism_name>'
echo ' Allows for specifiying the organism name in the embl generated file'
echo ' Spaces have to be substituted by underscore ex : Abies_alba'
echo
echo ' -c | --chloroplast'
echo ' Selects for the annotation of a chloroplast genome'
echo ' This is the default mode'
echo
echo ' -r | --nuclear-rdna'
echo ' Selects for the annotation of the rDNA nuclear cistron'
echo
echo ' -m | --mitochondrion'
echo ' Selects for the annotation of an animal mitochondrion genome'
exit $2
}
# options may be followed by one colon to indicate they have a required argument
if ! options=$(getopt -o t:o:ih -l ncbi-taxid:,organism,no-ir-detection,help -- "$@")
if ! options=$(getopt -o t:o:icrmh -l ncbi-taxid:,organism,no-ir-detection,chloroplast,nuclear-rdna,mitochondrion,help -- "$@")
then
# something went wrong, getopt will put out an error message for us
exit 1
......@@ -36,20 +68,13 @@ eval set -- "$options"
while [ $# -gt 0 ]
do
case $1 in
-t|--ncbi-taxid) taxid="$2" ; shift;;
-t|--ncbi-taxid) taxid="$2" ; shift ;;
-i|--no-ir-detection) irdetection="no" ;;
-o|--organism) organism="$2" ; shift;;
-h|--help) echo "Usage:" ;
echo " $0 "'[-t|--ncbi-taxid ###] [-n|--no-normalization] \'
echo " [-i|--no-ir-detection] [-h|--help] <FASTAFILE>"
echo
echo "Options:"
echo ' -t ### | --ncbi-taxid ###'
echo ' ### represents the ncbi taxid associated to the sequence'
echo
echo ' -i | --no-ir-detection'
echo ' Does not look for inverted repeats in the plastid genome'
exit 0;;
-o|--organism) organism="$2" ; shift ;;
-c|--chloroplast) types="chloro" ;;
-r|--nuclear-rdna) types="nucrdna" ;;
-m|--mitochondrion) types="mito" ;;
-h|--help) usage $0 0;;
(--) shift; break;;
(-*) echo "$0: error - unrecognized option $1" 1>&2; exit 1;;
(*) break;;
......@@ -57,6 +82,7 @@ do
shift
done
echo $type
#############################
pushTmpDir ORG.organnot
......@@ -73,43 +99,78 @@ pushTmpDir ORG.organnot
rm -f ${LOG}
openLogFile ${LOG}
if [ "$irdetection"=="yes" ]; then
loginfo "Normalizing the structure of the Chloroplast sequence..."
loginfo " LSC + IRB + SSC + IRA"
${PROG_DIR}/detectors/normalize/bin/go_normalize.sh ${QUERY} > "${RESULTS}.norm.fasta"
loginfo "Done."
case "$types" in
chloro)
loginfo "Annotating a plant chloroplast genome..."
if [ "$irdetection"=="yes" ]; then
loginfo "Annotating the Inverted repeats and Single copies (LSC and SSC)..."
${PROG_DIR}/detectors/ir/bin/go_ir.sh "${RESULTS}.norm.fasta" > "${RESULTS}.annot"
loginfo "Done."
loginfo "Normalizing the structure of the Chloroplast sequence..."
loginfo " LSC + IRB + SSC + IRA"
${PROG_DIR}/detectors/normalize/bin/go_normalize.sh ${QUERY} > "${RESULTS}.norm.fasta"
loginfo "Done."
loginfo "Annotating the Inverted repeats and Single copies (LSC and SSC)..."
${PROG_DIR}/detectors/ir/bin/go_ir.sh "${RESULTS}.norm.fasta" > "${RESULTS}.annot"
loginfo "Done."
fi
loginfo "Annotating the tRNA..."
${PROG_DIR}/detectors/trna/bin/go_trna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
loginfo "Done."
loginfo "Annotating the rRNA genes..."
${PROG_DIR}/detectors/rrna/bin/go_rrna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
loginfo "Done."
loginfo "Annotating the CDS..."
tcsh -f ${PROG_DIR}/detectors/cds/bin/go_cds.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
loginfo "Done."
topology="circular"
defline="plastid, complete genome"
;;
nucrdna)
loginfo "Annotating a plant rDNA cistron..."
loginfo "Normalizing the structure of the cistron sequence..."
${PROG_DIR}/detectors/normalizerdna/bin/go_normalizerdna.sh ${QUERY} > "${RESULTS}.norm.fasta"
loginfo "Done."
loginfo "Annotating the rRNA genes..."
${PROG_DIR}/detectors/nucrrna/bin/go_nucrrna.sh "${RESULTS}.norm.fasta" > "${RESULTS}.annot"
loginfo "Done."
topology="linear"
defline="18S rRNA gene, ITS1, 5.8S rRNA gene, ITS2 and 28S rRNA gene"
;;
mito)
loginfo "Annotating an animal mitochondrial genome..."
logerror "Not yet implemented"
topology="circular"
defline="mitochondrion, complete genome"
exit 1
;;
*)
echo usage $0 1;;
esac
if [[ "${organism}" == "no" ]]; then
organism="{organism}"
else
organism="$(echo ${organism} | tr '_' ' ')"
fi
loginfo "Annotating the tRNA..."
${PROG_DIR}/detectors/trna/bin/go_trna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
loginfo "Done."
loginfo "Annotating the rRNA genes..."
${PROG_DIR}/detectors/rrna/bin/go_rrna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
loginfo "Done."
loginfo "Annotating the CDS..."
tcsh -f ${PROG_DIR}/detectors/cds/bin/go_cds.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
loginfo "Done."
loginfo "Printing minimal header..."
echo "ID XXX; XXX; circular; genomic DNA; XXX; XXX; $(seqlength ${RESULTS}.norm.fasta) BP."
echo "ID XXX; XXX; ${topology}; genomic DNA; XXX; XXX; $(seqlength ${RESULTS}.norm.fasta) BP."
echo "XX"
echo "AC XXX;"
echo "DE ${organism} ${defline}."
echo "XX"
if [[ "${organism}" == "no" ]]; then
echo "DE {organism} plastid, complete genome."
else
echo "DE $(echo ${organism} | tr '_' ' ') plastid, complete genome."
fi
echo "XX"
loginfo "Done."
loginfo "Printing annotations header..."
......
......@@ -182,6 +182,12 @@ CDS_DATA_DIR="${DATA_DIR}/cds" # Directory containing data related to
RRNA_DATA_DIR="${DATA_DIR}/rrna" # Directory containing data related to
# rRNAs detection
NUCRRNA_DATA_DIR="${DATA_DIR}/nucrrna" # Directory containing data related to
# rRNAs detection
ITS_DATA_DIR="${DATA_DIR}/its" # Directory containing data related to
# rRNAs detection
#
#
......
......@@ -25,31 +25,44 @@ PRTPATH = $(abspath $(PRTDIR))
DATADIR = $(CFGDIR)../data
DATAITS = $(DATADIR)/its
HMMPRESS= $(BINDIR)/hmmpress
HMMDIR = $(PKGDIR)/ITSx_db/HMMs
HMMS = $(wildcard $(HMMDIR)/*.hmm)
HMMP = $(patsubst %.hmm,%.hmm.h3p,$(HMMS))
HMMI = $(patsubst %.hmm,%.hmm.h3i,$(HMMS))
HMMM = $(patsubst %.hmm,%.hmm.h3m,$(HMMS))
#
# Rules
#
.PHONY: all clean test portclean pkg pkg.expand pkg.install
all:: pkg
%.hmm.h3i: %.hmm
echo $(HMMPRESS)
(! test -s $< ) || $(HMMPRESS) -f $<
all:: pkg.install
pkg.expand::
test -d $(PKGDIR) || mkdir $(PKGDIR)
$(TAR) zxf $(PKGTAR) -C $(PKGDIR) --strip-components 1
pkg.install:: pkg.expand
pkg.install:: pkg
@mkdir -p $(BINDIR)
@cp $(PKGDIR)/ITSx $(BINDIR)
@mkdir -p $(DATAITS)
@cp -r $(PKGDIR)/ITSx_db $(DATAITS)
@echo "+++++++++++ package $(PKG) done"
pkg:: pkg.install
pkg:: pkg.expand clean $(HMMI)
test::
echo No test available
clean::
\rm -f $(HMMP) $(HMMI) $(HMMM)
echo Done
portclean::
......
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