... | @@ -55,11 +55,11 @@ And unzip it: |
... | @@ -55,11 +55,11 @@ And unzip it: |
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We can check the average alignment score (corresponding approximately to the similarity between the sequences in the overlap):
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We can check the average alignment score (corresponding approximately to the similarity between the sequences in the overlap):
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obi stats -a score_norm wolf/aligned_reads
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obi stats -a score_norm wolf/aligned_reads
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### 3. Remove unaligned sequence records
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### 3. Remove sequence records with a low overlap alignment score:
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obi grep -a mode:alignment wolf/aligned_reads wolf/good_sequences
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obi grep -p "sequence['score_norm'] > 0.8" wolf/aligned_reads wolf/good_sequences
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### 4. Assign each sequence record to the corresponding sample/marker combination
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### 4. Assign each sequence record to the corresponding sample/marker combination
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