Wolf-tutorial-with-the-OBITools3.md

@@ -49,6 +49,10 @@ And unzip it:
 	
 	obi alignpairedend -R wolf/reads2 wolf/reads1 wolf/aligned_reads
 
+**Note:** For any command, you can also print the result to standard output using the `-` URI:
+
+	obi alignpairedend -R wolf/reads2 wolf/reads1 - > aligned_reads.fastq
+
 ### 3. Remove unaligned sequence records
 
 	obi grep -a mode:alignment wolf/aligned_reads wolf/good_sequences
@@ -57,7 +61,7 @@ And unzip it:
 
 	obi ngsfilter -t wolf/ngsfile -u wolf/unidentified_sequences wolf/good_sequences wolf/identified_sequences
 
-Note: Unlike the OBITools1, the OBITools3 make it possible to run ngsfilter before aligning the paired-end reads, BUT it is not recommended to do so for usual data, as ngsfilter will not be able to detect and cut out partially sequenced primers.
+**Note:** Unlike the OBITools1, the OBITools3 make it possible to run ngsfilter before aligning the paired-end reads, BUT it is not recommended to do so for usual data, as ngsfilter will not be able to detect and cut out partially sequenced primers.
 	
 ### 5. Dereplicate reads into unique sequences